Stimulated Emission Depletion (STED) microscopy is a super-resolution imaging technique that overcomes the diffraction limit of conventional optical microscopy. It enables the visualization of ...

A decade ago, the Nobel Prize in Chemistry was awarded to a trio of researchers for the development of super-resolved fluorescence microscopy. The announcement at the time stated that the researchers’ ...

Researchers enhanced Stimulated Emission Depletion (STED) microscopy by replacing traditional fluorophores with nanographenes, enabling the observation of longer-duration processes, overcoming a ...

Then, using Time-gated STED Microscopy, the research team made still images at 60 nanometre resolution (about one thousandth of the width of a human hair), as well as time-lapse image sequences ...

Nanoscopy describes the ability to see beyond the generally accepted optical limit of 200–300 nm. Stimulated emission depletion (STED) microscopy, developed by Stefan W. Hell and Jan Wichmann in 1994, ...

Confocal and multiphoton microscopes are standard fare in academic imaging facilities, but ultrahigh-resolution systems generally have a smaller user base – namely, their inventors. Soon facilities ...

Both images show individual red blood cells. The one on the right, produced by STED, more clearly shows the Piezo1 proteins (in green), which are a mechanical calcium channel and play a crucial role ...

Novel developments also focus on quick and high-speed AFM to improve temporal resolution. 2 The combination of AFM with optical microscopy has expanded the number of possible applications, ...