Reverse Transcriptase | Function, Structure & Application
Learn the reverse transcriptase enzyme definition. Explore the functions and structure of reverse transcriptase, and discover which organisms have this enzyme.
The regulations of telomerase reverse transcriptase (TERT) in cancer
Jan 26, 2024 · Telomerase reverse transcriptase (TERT) is a key component of telomerase. Human TERT was first cloned in 1997 [5, 8, 9, 10], eight years after telomerase was first identified in HeLa …
The Biotechnology Revolution: PCR and the Use of Reverse …
Jun 27, 1970 · At this point, the reverse transcriptase enzyme is added, and this enzyme proceeds to utilize the mRNA strand as a template for the synthesis of a complementary DNA strand.
Discovery and evolution of RNA and XNA reverse transcriptase function ...
Jul 20, 2020 · The ability of reverse transcriptases (RTs) to synthesize a complementary DNA from natural RNA and a range of unnatural xeno nucleic acid (XNA) template chemistries, underpins key …
Reverse transcription-quantitative PCR (RT-qPCR) without the need for ...
Jul 15, 2023 · For all samples, negative controls without reverse transcriptase enzyme were also prepared. Quantitative real-time PCR (qPCR) for satellite DNA expression analysis
The reverse transcriptase domain of prime editors contributes to DNA ...
Feb 17, 2025 · Reverse transcriptase (RT) has been shown to play a role in double-strand break repair in bacteria, yet the impact of the RT component of prime editors (PEs) on normal mammalian cellular …
Protein-primed homopolymer synthesis by an antiviral reverse …
May 28, 2025 · A bacterial reverse transcriptase synthesizes polydeoxyadenylate to provide population-level immunity against viral infection.
Structural basis for pegRNA-guided reverse transcription by a ... - Nature
May 29, 2024 · The prime editor system composed of Streptococcus pyogenes Cas9 nickase (nSpCas9) and engineered Moloney murine leukaemia virus reverse transcriptase (M-MLV RT) collaborates with …
Regulation of human telomerase in homeostasis and disease
Apr 2, 2020 · Telomere length is maintained by telomerase, which comprises a reverse transcriptase and a template RNA. Telomerase activity is disrupted in several genetic disorders, but is commonly …
High-resolution view of HIV-1 reverse transcriptase initiation ...
May 4, 2021 · Initiation of HIV-1 reverse transcription occurs at the host tRNALys3, which forms a complex with the 5’ end of the HIV-1 viral RNA and reverse transcriptase (RT). Here, the authors …